| 胡文杰,俞静怡,罗辉,卓志嘉,周兵,彭舒军,殷帅文.α-红没药醇抑制油茶炭疽病病菌的机理研究[J].井冈山大学自然版,2025,(5):62-68 |
| α-红没药醇抑制油茶炭疽病病菌的机理研究 |
| THE MECHANISM OF α-BISABOLOL IN INHIBITING ANTHRACNOSE IN CAMELLIA OLEIFERA |
| 投稿时间：2025-04-16 修订日期：2025-05-20 |
| DOI：10.3969/j.issn.1674-8085.2025.05.008 |
| 中文关键词: α-红没药醇 油茶炭疽病防治 胶孢炭疽菌 抑菌机理 |
| 英文关键词: α-bisabolol control of anthracnose of Camellia oleifera Colletotrichum gloeosporioides antifungal mechanism |
| 基金项目:国家自然科学基金项目(32160089); 江西省自然科学基金资助面上项目(20232BAB205044,20181BAB204014,20252BAC240486); 江西省教育厅科技研究重点项目(GJJ2201609); 国家级大学生创新训练计划项目(202510419008) |
| 作者 | 单位 | E-mail | | 胡文杰 | 井冈山大学生命科学学院, 江西, 吉安 343009 | huwenjie2008@126.com | | 俞静怡 | 井冈山大学生命科学学院, 江西, 吉安 343009 | | | 罗辉 | 井冈山大学基础医学院, 江西, 吉安 343009 | | | 卓志嘉 | 井冈山大学基础医学院, 江西, 吉安 343009 | | | 周兵 | 井冈山大学生命科学学院, 江西, 吉安 343009 | | | 彭舒军 | 井冈山大学化学化工学院, 江西, 吉安 343009 | | | 殷帅文 | 井冈山大学生命科学学院, 江西, 吉安 343009 | |
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| 中文摘要: |
| 本研究以α-红没药醇为研究对象，通过开展油茶炭疽病菌菌丝生长抑制试验，并结合扫描电镜观察菌丝形态变化、电导率法测定细胞膜通透性等指标分析，深入探究其抑菌机理。研究结果表明，该化合物最低抑菌浓度（MIC）为0.050 00μL/mL，可通过多途径协同作用抑制病原菌：电导率测定显示其破坏细胞膜完整性（12 h电导率提高5.289 70倍），扫描电镜观察到菌丝严重皱缩变形；同时干扰氧化应激系统（其SOD活性先升高至对照3.186 00倍后急剧下降，CAT活性持续升高至1.865 30 U/mg prot）；并显著影响菌丝代谢功能（可溶性蛋白泄漏量为对照组的3.584 10倍，可溶性糖含量降低，Ca++Mg++-ATP酶活性下降93.4%）。由此可见，α-红没药醇可通过破坏细胞膜结构、扰乱氧化平衡和抑制能量代谢等多靶点机制，进而发挥其抑菌作用，这为研发用于防控油茶炭疽病的植物源杀菌剂提供理论支撑。 |
| 英文摘要: |
| This study took α-bisabolol as the research object. Through conducting the mycelial growth inhibition test of the Colletotrichum gloeosporioides（the pathogen causing anthracnose of Camellia. oleifera）, and combining with the analysis of indicators such as observing the morphological changes of mycelia by scanning electron microscopy and measuring the cell membrane permeability by the electrical conductivity method, the antibacterial mechanism was explored in depth. The results showed that the minimum inhibitory concentration（MIC） of this compound was 0.050 00 μL/mL, and it could inhibit the pathogenic bacteria through the synergistic effect of multiple pathways. Electrical conductivity increased 5.289 70-fold after 12 h, indicating the damage of cell membrane, while scanning electron microscopy revealed severe mycelial shrinkage and deformation; the oxidative stress system was disrupted as superoxide dismutase（SOD） activity peaked at 3.186 00 times the control then declined sharply, and catalase（CAT） activity increased to 1.865 30 U/mg prot; metabolic function was significantly affected with soluble protein leakage 3.584 10-fold higher than the control, reduced soluble sugar content, and a 93.4% decrease in Ca++Mg++-ATPase activity. Therefore, α-bisabolol can exert antibacterial effects through multiple-target mechanisms such as damaging cell membrane structure, disrupting oxidative balance, and inhibiting energy metabolism, it would provide a theoretical support for the development of plant-derived bactericides for the prevention and control anthracnose of C. oleifera. |
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