| 胡文海,曾文悦,王佳慧,罗嘉欣.低温弱光和水杨基氧肟酸处理对番茄幼苗叶绿素荧光成像特性的影响[J].井冈山大学自然版,2025,46(4):48-55 |
| 低温弱光和水杨基氧肟酸处理对番茄幼苗叶绿素荧光成像特性的影响 |
| EFFECTS OF LOW TEMPERATURE WITH LOW LIGHT AND SHAM TREATMENT ON CHLOROPHYLL FLUORESCENCE IMAGING CHARACTERISTICS OF TOMATO SEEDLINGS |
| 投稿时间：2025-01-21 修订日期：2025-03-12 |
| DOI：10.3969/j.issn.1674-8085.2025.04.006 |
| 中文关键词: 番茄 低温弱光 PSII功能 光抑制 叶绿素荧光成像 |
| 英文关键词: Solanum lycopersicum L. low temperature with low light PSII function photoinhibition chlorophyll fluorescence imaging |
| 基金项目:国家自然科学基金项目(32460752)；井冈山大学校级大学生创新创业训练计划项目(JDX2024177) |
| 作者 | 单位 | E-mail | | 胡文海 | 井冈山大学生命科学学院, 江西, 吉安 343009
生物入侵与生物安全江西省重点实验室, 江西, 吉安 343009 | huwenhai@jgsu.edu.cn | | 曾文悦 | 井冈山大学生命科学学院, 江西, 吉安 343009 | | | 王佳慧 | 井冈山大学生命科学学院, 江西, 吉安 343009 | | | 罗嘉欣 | 井冈山大学生命科学学院, 江西, 吉安 343009 | |
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| 中文摘要: |
| 本研究旨在探究线粒体交替氧化酶（AOX）途径在缓解低温弱光胁迫下番茄幼苗光抑制中的作用。以番茄品种中蔬4号（Solanum lycopersicum L.cv Zhongshu No.4）为材料，分析了水杨基氧肟酸（SHAM,1.0 mM）对低温弱光（8℃、200 μmol·m-2·s-1）处理4 d的番茄幼苗叶绿素荧光成像特性的影响。结果表明，低温弱光导致番茄幼苗光抑制现象的发生，且对老叶的影响大于新生叶。低温弱光显著降低了Y(II)和qP的荧光活性区域相对面积（RAF）和全株荧光值（VW），但增加了qP的荧光活性区域荧光值（VA），以及Y (NPQ)的VW和VA。SHAM处理并未对常温下的番茄幼苗叶绿素荧光成像特性造成影响。但是，SHAM处理加剧了低温弱光处理下番茄幼苗Fv/Fm的下降，并增加了重度光抑制区域的分布比例。SHAM处理也加剧了低温弱光处理下番茄Y (II)和qP的RAF和VW下降，以及qP的VA增加，但Y(NPQ)的VW和VA有所下降。由此可见，低温弱光诱导了番茄幼苗部分PSII反应中心的关闭，并抑制了PSII光化学反应；AOX途径在缓解番茄幼苗低温光抑制，优化PSII反应中心开放程度与光化学反应，以及稳定热耗散途径等方面具有重要作用。 |
| 英文摘要: |
| The aim of this study was to explore the role of the mitochondrial alternative oxidase (AOX) pathway in alleviating photoinhibition in tomato seedlings subjected to low temperature with low light. In this study, Solanum lycopersicum L.cv Zhongshu No.4 was used as the research material, and the effects of salicylhydroxamic acid (SHAM, 1.0 mM) on the chlorophyll fluorescence imaging characteristics of tomato seedlings under low temperature with low light (LT, 8 °C, 200 μmol·m-2·s-1) for 4 d were analyzed. The results showed that LT treatment induced the photoinhibition in tomato seedlings, and its impact on older leaves was greater than that on newly born leaves. LT treatment significantly decreased the relative area of the fluorescence active regions (RAF) and the fluorescence values of whole plant (VW) of Y(II) and qP, but increased the fluorescence values of the fluorescence activity regions (VA) of qP, and also significantly promoted an increase in the VW and VA of Y(NPQ) in tomato seedlings. SHAM treatment did not affect the chlorophyll fluorescence imaging characteristics of tomato seedlings under normal temperature. However, SHAM treatment exacerbated the decrease of Fv/Fm and increased the distribution proportion of the severe photoinhibition region in tomato seedlings under low temperature with low light. SHAM treatment also aggravated the decrease in the RAF and VW of Y(II) and qP, and the increase in the VA of qP in tomato seedlings under low temperature with low light, while the VW and VA decreased slightly. These results implied that low temperature with low light induced the closure of some active PSII reaction centers, and inhibited the PSII photochemistry. AOX pathway played a critical role in alleviating photoinhibition, optimizing the opening degree of the PSII reaction centers and the PSII photochemistry, as well as stabilizing the thermal dissipation pathway in tomato seedlings under low temperature with low light conditions. |
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