| 赵珊珊,肖永红,程涛,徐湘,刘继贺,梁建辉.吗啡对阴暗拟隙蛛（蜘蛛目：漏斗蛛科）自主活动和探索行为的影响[J].井冈山大学自然版,2025,46(3):53-60 |
| 吗啡对阴暗拟隙蛛（蜘蛛目：漏斗蛛科）自主活动和探索行为的影响 |
| MORPHINE EXPOSURE AFFECTS LOCOMOTOR ACTIVITY AND EXPLORATORY BEHAVIOR OF THE FUNNEL-WEB SPIDER PIRENEITEGA LUCTUOSA |
| 投稿时间：2025-01-06 修订日期：2025-03-06 |
| DOI：10.3969/j.issn.1674-8085.2025.03.008 |
| 中文关键词: 漏斗蛛 自主活动 探索行为 阿片受体激动剂 吗啡 |
| 英文关键词: Agelenidae open field test exploratory behavior opioid receptor agonist morphine |
| 基金项目:国家自然科学基金项目(32160243,81773705)；江西省教育厅科技研究重点项目(GJJ190543) |
| 作者 | 单位 | E-mail | | 赵珊珊 | 井冈山大学生命科学学院, 江西, 吉安 343009 | | | 肖永红 | 井冈山大学生命科学学院, 江西, 吉安 343009 | | | 程涛 | 北京大学药学院, 北京 100191 | | | 徐湘 | 湖南师范大学生命科学学院, 湖南, 长沙 410081 | | | 刘继贺 | 井冈山大学生命科学学院, 江西, 吉安 343009
湖南师范大学生命科学学院, 湖南, 长沙 410081 | liujihe@jgsu.edu.cn | | 梁建辉 | 北京大学药学院, 北京 100191 | liangjh@pku.edu.cn |
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| 中文摘要: |
| 蜘蛛是低等动物中一种行为复杂且独特的无脊椎动物，是研究动物行为的理想动物。阿片受体激动剂吗啡因其多种神经精神药理活性，能够作用于动物中枢神经系统，显著影响动物行为。然而，目前关于吗啡的研究主要集中于高等动物，鲜有对于低等动物的行为影响的研究。本研究利用SMART 3.0行为追踪软件，记录吗啡处理后阴暗拟隙蛛（Pireneitega luctuosa）在旷场环境中的自主行为特点，分析吗啡对阴暗拟隙蛛自主行为活跃性及对未知区域探索行为的影响，研究结果显示：（1）未经处理、去离子水处理及各种浓度吗啡处理后的阴暗拟隙蛛，在旷场中的自主活动距离均呈现前高后低的特点，即监测前期行为活跃后期趋于安静；（2）吗啡处理后90 min内阴暗拟隙蛛的自主活动距离减少，且活动距离与吗啡浓度呈负相关；（3）各浓度吗啡处理均减少了阴暗拟隙蛛对中央区域的探索时间和距离，显著增强了其在边缘区域的活动倾向，尤其高浓度处理组与对照组差异明显。综上，吗啡不仅显著抑制了阴暗拟隙蛛在短时间内的行为活跃度，还降低了其对未知区域的探索能力。该作用的药理学机制仍需进一步研究。 |
| 英文摘要: |
| Spiders are invertebrates with complex and unique behaviors, making them ideal model organisms for studying animal behavior. The opioid receptor agonist morphine exhibits various neuropsychopharmacological activities, acting on the central nervous system and significantly influencing animal behavior. However, research on the effects of morphine has primarily focused on higher animals, with little attention given to its impact on the behavior of lower animals. The current study aims to investigate how morphine exposure affects locomotor activity and exploratory behavior of the funnel-web spider Pireneitega luctuosa in the open field environment by using the behavioral tracker software. Our results showed that all the spiders, including the untreated, distilled water treated (control) and morphine treated spiders, moved vigorously when they were just introduced into the open field container, then slowed down their speed and became inactive over time. The preference of staying in the peripheral zone of the container over the central zone was also displayed by the spiders. However, the distance of locomotor activity of the spider P. luctuosa was negatively correlated with the morphine exposure concentration within 90 min of the monitoring period. Furthermore, both of the duration and distance of exploration to the central zone dropped down after the spiders being sprayed with the morphine solutions and significant differences being detected between the control and 60 mg/mL-morphine-treated group. Therefore, our study suggests that the morphine exposure may produce a negative effect on the locomotor activity and exploratory behavior in the funnel-web spider P. luctuosa. |
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