| 宁静华,张鑫,赵严红,屈润,张钰哲.银屑病和黑色素瘤共表达的核心基因筛选及潜在合并症机制分析[J].井冈山大学自然版,2024,45(6):66-76 |
| 银屑病和黑色素瘤共表达的核心基因筛选及潜在合并症机制分析 |
| SCREENING OF HUB GENES CO-EXPRESSED IN PSORIASIS AND MELANOMA AND ANALYSIS OF POTENTIAL COMORBIDITY MECHANISMS |
| 投稿时间：2024-08-22 修订日期：2024-09-26 |
| DOI：10.3969/j.issn.1674-8085.2024.06.010 |
| 中文关键词: 银屑病 黑色素瘤 差异表达基因 转录因子 两病同治 |
| 英文关键词: psoriasis melanoma differentially expressed genes transcription factors combined treatment of psoriasis and melanoma |
| 基金项目:国家自然科学基金项目(81860742)；云南省地方高校联合专项面上项目(202001BA070001-064，202101BA070001-102)；云南省教育厅科学研究基金项目(2023Y0950；2022Y80)；云南省昆虫生物医药研发重点实验室开放项目(AG2024002，AP2024015，AP2024016)；大理大学博士科研启动基金项目(KYBS2018012) |
| 作者 | 单位 | E-mail | | 宁静华 | 大理大学基础医学院, 云南, 大理 671000 | | | 张鑫 | 大理大学基础医学院, 云南, 大理 671000 | | | 赵严红 | 大理大学基础医学院, 云南, 大理 671000 | | | 屈润 | 大理大学基础医学院, 云南, 大理 671000 | | | 张钰哲 | 大理大学基础医学院, 云南, 大理 671000
云南省昆虫生物医药重点实验室, 云南, 大理 671000
云南抗病原药用植物筛选重点实验室, 云南, 大理 671000 | lzuzyz1568@hotmail.com |
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| 中文摘要: |
| 本研究应用生物信息学方法筛选银屑病和黑色素瘤共同表达的核心基因和分子机制，为后续研究提供新思路。从基因表达综合数据库下载黑色素瘤基因表达数据集（GSE7553、GSE15605）和银屑病基因表达数据集（GSE14905、GSE30999），鉴定出共同差异表达基因后进行GO和KEGG富集分析，使用STRING数据库和Cytoscape软件构建蛋白质-蛋白质相互作用网络并筛选核心基因。随后，构建核心基因共表达网络以及进行GO、KEGG富集分析。使用GSE15605数据集和GSE30999数据集对筛选出的核心基因进行验证。最后，通过核心基因构建转录因子调控网络。本研究共筛选出10个核心基因：MMP9、COKN1A、ADIPOQ、FEN1、KRT19、DTL、TPX2、TYMS、PCK1和PIP。GO富集分析显示，核心基因功能主要与紫外线反应、DNA生物合成、光刺激等有关；KEGG富集分析显示，核心基因主要参与膀胱癌、脂肪因子信号通路、PPAR信号通路等代谢通路。RELA和NFKB1为调控核心基因的关键转录因子。本研究初步揭示了银屑病和黑色素瘤的合并发病分子机制，为银屑病和黑色素瘤“两病同治”的思路提供理论依据。 |
| 英文摘要: |
| Bioinformatics methods were used to screen the hub genes and analyze the molecular mechanisms co-expressed by psoriasis and melanoma, providing new ideas for follow-up studies. The melanoma gene expression datasets (GSE7553, GSE15605) and psoriasis gene expression datasets (GSE14905, GSE30999) were downloaded from the Gene Expression Omnibus database, and the co-expressed genes were identified and analyzed by GO and KEGG enrichment, and protein-protein interaction networks were constructed to screen the hub genes using the STRING database and Cytoscape software. Subsequently, the hub gene co-expression network was constructed and GO and KEGG enrichment analysis was performed. The screened hub genes were validated using GSE15605 dataset and GSE30999 dataset. Finally, transcription factor regulatory networks were constructed from the hub genes. A total of 10 hub genes were screened in this study: MMP9, COKN1A, ADIPOQ, FEN1, KRT19, DTL, TPX2, TYMS, PCK1, and PIP. GO enrichment analysis showed that the hub genes' functions were mainly related to ultraviolet response, DNA biosynthesis, and photostimulation, etc., and KEGG enrichment analysis showed that the hub genes were mainly involved in bladder cancer, adipokine signaling pathway, PPAR signaling pathway and other metabolic pathways; RELA and NFKB1 are key transcription factors regulating the hub genes. This study initially reveals the molecular mechanism of the combined pathogenesis of psoriasis and melanoma, and provides a theoretical basis for the idea of “two-disease simultaneous treatment” of psoriasis and melanoma. |
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