| 刘红梅,孙传伯.犀角地黄汤预防小鼠中暑效果及血液指标的变化[J].井冈山大学自然版,2024,45(1):61-67 |
| 犀角地黄汤预防小鼠中暑效果及血液指标的变化 |
| EFFECTS OF XIJIAO DIHUANG DECOCTION IN PREVENTING HEAT STROKE IN MICE AND THE CHANGES OF HAEMATOLOGICAL INDEX |
| 投稿时间：2023-07-16 修订日期：2023-10-19 |
| DOI：10.3969/j.issn.1674-8085.2024.01.001 |
| 中文关键词: 犀角地黄汤 中暑 血液指标 |
| 英文关键词: Xijiao Dihuang decoction heat stroke haematological index |
| 基金项目:国家自然科学基金项目(81872955)；安徽省高校自然科学基金重点项目（2022AH051668） |
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| 中文摘要: |
| 为了探究犀角地黄汤对中暑小鼠的预防效果及血液指标变化的影响。将30只小鼠随机分为对照组、中暑组和干预组，每组10只，干预组小鼠每天使用11.375 g/kg犀角地黄汤进行灌胃，连续用药5 d，对照组和中暑组小鼠灌胃等量的生理盐水。在最后一次灌胃后2 h进行中暑造模。将中暑组和干预组小鼠置于40℃的恒温环境中持续60 min，对照组小鼠置于室温环境中，对比各组小鼠的抗中暑效果，试验结束后，小鼠眼眶分别采集抗凝血用于血常规检测，取血清用于血清生化和ELISA检测。结果显示，与空白组相比，中暑组小鼠的肛温显著升高(P < 0.05)，与中暑组相比，干预组小鼠肛温显著下降(P < 0.05)；与空白组相比，中暑组小鼠血液WBC、Gran#、Lymph#、Mon#、RBC、HGB、HCT、MCV、RDW、MPV、PDW水平显著升高(P < 0.05)，PLT、PCT水平显著下降(P < 0.05)，与中暑组相比，干预组小鼠血液WBC、Gran#、Lymph#、Mon#、RBC、HGB、HCT、MCV、MPV、PDW显著下降(P < 0.05)，PLT、PCT水平显著升高(P < 0.05)；与空白组相比，中暑组小鼠血清AST、ALT、BUN、CREA和CK含量显著升高(P < 0.05)，与中暑组相比，干预组小鼠血清AST、ALT、BUN、CREA、CK显著下降(P < 0.05)；与空白组相比，中暑组小鼠血清MDA、TNF-α、IL-6和IL-1β含量显著升高(P < 0.05)，SOD、GSH-PX和HSP70显著下降(P < 0.05)，与中暑组相比，干预组血清MDA、TNF-α、IL-6和IL-1β含量显著下降(P < 0.05)，HSP70、SOD和GSH-PX含量显著升高(P < 0.05)。表明犀角地黄汤可以有效地预防小鼠中暑疾病的发生，其机制可能与抑制炎性反应和提高机体的抗氧化水平有关，可为犀角地黄汤用于中暑病的防治提供一定的理论依据。 |
| 英文摘要: |
| In order to investigate the effects of Xijiao Dihuang decoction on the prevention of heat stroke in mice and its changes of haematological index. Thirty mice were randomized to blank group, heat stroke group and intervention group, 10 mice in each group. Mice in the intervention group were gavaged with 11.375 g/kg of Xijiao Dihuang decoction for 5 days, and mice in the blank and heat stroke groups were gavaged with equal amounts of saline. And mice were given equal doses of Xijiao Dihuang decoction for gavage 2 h before moulding. The mice in the heat stroke and intervention groups were placed at a constant temperature of 40 ℃ for 60 min, while the mice in the blank group were placed at room temperature to compare the anti-stroke effect of each group. The results showed that the anal temperature of mice in the heatstroke group was significantly increased compared with the blank group (P < 0.05), which was significantly decreased in the intervention group compared with the heatstroke group (P < 0.05); the levels of blood WBC, Gran#, Lymph#, Mon#, RBC, HGB, HCT, MCV, RDW, MPV, and PDW of mice in the heatstroke group were significantly increased compared with the blank group (P < 0.05), which were significantly decreased in the intervention group compared with the heatstroke group (P < 0.05), it was reversed for PLT and PCT levels. Compared with the blank group, serum AST, ALT, BUN, CREA, and CK levels were significantly increased in the heatstroke group of mice (P < 0.05), which were significantly decreased in the intervention group of mice compared with the heatstroke group (P < 0. 05). Serum MDA, TNF-α, IL-6 and IL-1β levels were significantly increased in the heatstroke group of mice compared with the blank group (P < 0.05), and SOD, GSH-PX and HSP70 were significantly decreased (P < 0.05). The serum levels of MDA, TNF-α, IL-6 and IL-1β were significantly decreased (P < 0.05) and the levels of HSP70, SOD and GSH-PX were significantly increased (P < 0.05) in the intervention group compared with the heatstroke group. These suggests that Xijiao Dihuang decoction can effectively prevent the occurrence of heat stroke diseases, and its mechanism may be related to inhibiting the inflammatory response and increasing the antioxidant level, which may provide a certain theoretical basis for the use of Xijiao Dihuang decoction in the prevention and treatment of heat stroke diseases. |
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