| 王昱,何九军.白肉灵芝水提物对新生大鼠胆红素脑病的影响[J].井冈山大学自然版,2023,44(5):64-72 |
| 白肉灵芝水提物对新生大鼠胆红素脑病的影响 |
| EFFECT OF GANODERMA LEUCOCONTEXTUM AQUEOUS EXTRACTS ON NEONATAL RATS WITH BILLIRUBIN ENCEPHALOPATHY |
| 投稿时间：2022-09-24 修订日期：2023-06-17 |
| DOI：10.3969/j.issn.1674-8085.2023.05.010 |
| 中文关键词: 白肉灵芝水提物 胆红素脑病 抗氧化 神经营养 炎症 细胞凋亡 新生大鼠 |
| 英文关键词: Ganoderma leucocontextum aqueous extracts bilirubin encephalopathy antioxidant neurotrophy inflammation apoptosis neonatal rat |
| 基金项目:国家自然科学基金项目(32160055)；甘肃省科技重大专项 (21ZD4NK045)；甘肃省陇南市科技计划项目(2019-ZD-10) |
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| 中文摘要: |
| 为了研究白肉灵芝水提物（ganoderma leucocontextum aqueous extracts, GLAE）对新生大鼠胆红素脑病的改善作用。腹腔注射胆红素建立高胆红素血症大鼠模型，将新生大鼠分成对照组、模型组、GLAE低剂量组、GLAE中剂量组和GLAE高剂量组，分别给予不同剂量的（0、50、100、200 mg/kg）GLAE处理。利用试剂盒检测血液样本和大脑组织中总胆红素水平，比色法检测大脑组织ATP酶、超氧化物歧化酶（superoxide dismutase, SOD）、过氧化氢酶（catalase, CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase, GSH-Px）活性和丙二醛（malondialdehyde, MDA）含量，酶联免疫吸附（ELISA）法检测血清肿瘤坏死因子-α（tumornecrosisfactor-α，TNF-α）、白细胞介素-1β（interleukin-1β，IL-1β）、神经特异性烯醇化酶（neuron specific enolase, NSE）和中枢神经特异性蛋白（central nerve specific protein, S100β）的含量，实时荧光定量PCR和western blot检测大脑组织B淋巴细胞瘤-2（B-cell lymphoma-2, Bcl-2）、半胱氨酸天冬氨酸蛋白酶-3 （cysteine aspartate protease 3, Caspase-3）、B淋巴细胞瘤-2 相关X（Bcl-2 assaciated X, Bax）、脑源性神经生长因子（brain derived neurotrophic factor, BDNF）、神经生长因子（nerve growth factor, NGF）mRNA水平和蛋白含量。结果显示，与模型组相比，经GLAE干预后，大鼠血清及大脑组织胆红素浓度明显降低（P < 0.05，P < 0.01）；大脑组织SOD、CAT、GSH-Px、Na+-K+-ATP和Ca2+-ATP酶活性显著升高（P < 0.05，P < 0.01），MDA含量明显降低（P < 0.05，P < 0.01）；血清TNF-α、IL-1β、NSE、S100β含量显著降低（P < 0.05，P < 0.01）；大脑组织Caspase-3、Bax mRNA水平和蛋白含量明显降低（P< 0.05，P < 0.01），Bcl-2、BDNF、NGF mRNA水平和蛋白含量明显升高（P < 0.05，P < 0.01）。以上结果表明GLAE能减轻过量胆红素对新生大鼠的大脑损伤，其作用机制可能通过改善新生大鼠大脑能量代谢，提高大脑抗氧化能力，增加神经营养因子含量，抑制炎症反应及凋亡基因的表达来发挥作用。 |
| 英文摘要: |
| To explore the improvement effect of Ganoderma leucocontextum aqueous extracts (GLAE) on neonatal rats with bilirubin encephalopathy. A hyperbilirubinemia model of nenonatal rats was established by intraperitoneal injection of bilirubin. After successful model establishment, the rats were divided into five groups: control group, model group, GLAE low-dose group, GLAE middle-dose group and GLAE high-dose group, and given GLAE at doses of 0, 50, 100, 200 mg/kg by intragastric administration. The contents of bilirubin in serum and cerebral tissue of rats were detected by kit. Colorimetry was used to detect the activities of ATPase, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) contents in cerebral tissue. And the contents of tumornecrosisfactor-α (TNF-α), interleukin-1β (IL-1β), neuron specific enolase (NSE) and central nerve specific protein (S100β) in serum were detected by ELISA. Quantitative PCR and western blot were used to detect mRNA and protein content changes of B-cell lymphoma-2 (Bcl-2), cysteine aspartate protease 3(Caspase-3), Bcl-2 assaciated X (Bax), brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in cerebral tissue. Results showed that compared with the model group, GLAE administration could obviously decrease the bilirubin contents in serum and cerebral tissue to varying degrees, significantly activate the activities of SOD, CAT, GPx, Na+-K+-ATP and Ca2+-ATPase (P < 0.05, P < 0.01), while reduce the contents of MDA in cerebral tissue (P < 0.05,P < 0.01). Moreover, the contents of TNF-α, IL-1β, NSE and S100β in serum increased (P < 0.05, P < 0.01). The mRNA levels and protein contents of Caspase-3 and Bax decreased (P < 0.05, P< 0.01), meanwhile increased for Bcl-2, BDNF and NGF in cerebral tissue (P < 0.05, P < 0.01). The results of the present study indicated that GLAE could reduce the cerebral damage of excess bilirubin on new born rats. Its mechanism may be related to improving the cerebral energy metabolism, increasing the cerebral antioxidant capacity and neurotrophic factor content, inhibit expression of inflammatory response and apoptosis genes. |
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