| 焦春香,庞博,陈俊雅,耿玲,蓝海,周萍.响应面法优化红外辅助提取紫地榆总鞣质工艺[J].井冈山大学自然版,2023,44(4):30-36 |
| 响应面法优化红外辅助提取紫地榆总鞣质工艺 |
| OPTIMIZATION OF INFRARED ASSISTED EXTRACTION PROCESS FOR TOTAL TANNIN FROM GERANIUM STRICTIPES BY RESPONSE SURFACE METHODOLOGY |
| 投稿时间：2022-12-27 修订日期：2023-04-16 |
| DOI：10.3969/j.issn.1674-8085.2023.04.006 |
| 中文关键词: 紫地榆 提取工艺 响应面法 鞣质 红外辅助提取 |
| 英文关键词: Geranium strictipes extraction process response surface method tannin infrared-assisted extraction |
| 基金项目:国家自然科学基金项目（81260512）；大理大学教育部自然资源药物化学重点实验室开放基金项目(2021KF03) |
| 作者 | 单位 | | 焦春香 | 大理大学药学院, 云南, 大理 671000 | | 庞博 | 大理大学药学院, 云南, 大理 671000
云南大学教育部自然资源药物化学重点实验室, 云南, 昆明 650091 | | 陈俊雅 | 大理大学药学院, 云南, 大理 671000 | | 耿玲 | 大理大学基础医学院, 云南, 大理 671000 | | 蓝海 | 大理大学药学院, 云南, 大理 671000 | | 周萍 | 大理大学药学院, 云南, 大理 671000
云南大学教育部自然资源药物化学重点实验室, 云南, 昆明 650091 |
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| 中文摘要: |
| 采用红外辅助提取紫地榆有效成分鞣质，高效液相色谱法同时检测6种鞣质成分含量，以提取时间、料液比、乙醇浓度为自变量进行3因素3水平的中心组合试验。响应面法分析得到优化提取工艺为提取1次，乙醇浓度50 %，提取时间14 min，料液比1:150 g/mL，红外灯275 W，灯距1 cm。经该工艺提取，8批药材平均鞣质总含量为79.06 mg/ g，与预测值80.10 mg/ g相一致（P > 0.05）。优化的红外辅助提取工艺稳定、可靠，为紫地榆总鞣质的有效提取提供理论依据。 |
| 英文摘要: |
| To optimize the extraction process for total tannin from Geranium strictipes (T-Gs), infrared assisted extraction was employed and coupled with HPLC for simultaneous determination of six compounds of T-Gs. Three-factor, three-level Box-Behnken design was used to optimize some process parameters. The most critical factors affecting the extraction of T-Gs were extraction time, material-to-liquid ratio, ethanol concentration. The optimal infrared extraction conditions for T-Gs were as following: extraction time 14 min, material-to-liquid ratio 1:150 g/mL, ethanol concentration 50 %, infrared lamp height from round bottom flask 1 cm, infrared lamp power 275 W, extraction times 1 time. Under the optimal conditions, the average extraction yield of total tannin from 8 batches of medicinal materials was 79.06 mg/g, which was consistent with the theoretical extraction yield of 80.10 mg/g (P > 0.05). The optimized infrared assisted extraction process for T-Gs is stable and reliable, which provided a theoretical basis for the effective extraction of T-Gs. |
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