| 刘斌,余子琦,闫萌,戴彩华,张玮,叶紫辰,傅强.多重耐药UPEC细菌毒力基因分布特征及进化分型分析[J].井冈山大学自然版,2022,43(6):40-49 |
| 多重耐药UPEC细菌毒力基因分布特征及进化分型分析 |
| DISTRIBUTION CHARACTERISTICS OF VIRULENCE GENES AND EVOLUTIONARY TYPING OF MULTIDRUG-RESISTANT UPEC ISOLATES |
| 投稿时间：2022-07-06 修订日期：2022-10-15 |
| DOI：10.3969/j.issn.1674-8085.2022.06.007 |
| 中文关键词: UPEC 多重耐药 毒力因子 分布特征 进化分型 |
| 英文关键词: UPEC multi-drug resistance virulence factor distribution characteristics evolutionary typing |
| 基金项目:国家自然科学基金项目(31860711, 52163015);国家级大学生创新创业训练计划项目(202210419006);吉安市指导性科技计划项目[吉市科计字(2022)6号6] |
| 作者 | 单位 | | 刘斌 | 井冈山大学医学部, 江西, 吉安 343009 | | 余子琦 | 井冈山大学医学部, 江西, 吉安 343009 | | 闫萌 | 井冈山大学医学部, 江西, 吉安 343009 | | 戴彩华 | 井冈山大学医学部, 江西, 吉安 343009 | | 张玮 | 井冈山大学医学部, 江西, 吉安 343009 | | 叶紫辰 | 井冈山大学医学部, 江西, 吉安 343009 | | 傅强 | 井冈山大学医学部, 江西, 吉安 343009
井冈山大学脊柱疾病研究所, 江西, 吉安 343009 |
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| 中文摘要: |
| 本研究对临床尿路感染病例病原菌耐药情况、毒力基因分布及其进化分型特征，以期为耐药性尿路感染病原防控提供参考。通过对收集的尿样进行细菌分离培养，结合革兰氏染色镜检、16S rDNA对细菌进行鉴定，利用K-B纸片扩散法对细菌进行药敏试验，并通过PCR扩增进行毒力基因及系统发育和进化分型分析。本研究成功检测出20株尿路致病性大肠杆菌（Uropathogenic Escherichia coli, UPEC），药敏试验显示多数菌株具有多重耐药特征，该批UPEC对青霉素、红霉素、万古霉素耐药率达100%；对羧苄西林、氨苄西林、阿奇霉素耐药率达90%以上；对四环素、诺氟沙星、链霉素、耐药率达80%以上；对环丙沙星、 庆大霉素、卡那霉素、氟氧沙星、头孢曲松耐药率达70%以上；对头孢噻肟、呋喃妥因、氯霉素耐药率在35%以上；对阿莫西林与多粘菌素B耐药率低，分别仅为10%与5%。六个毒力基因（iutA、fimH、hly、fyuA、tsh、traT）检出率分别为85%、95%、85%、75%、25%、10%。其系统发育分型中，归属B2群的细菌最多，占比40%（n=8）；D群次之，占比35%(n=7);其次为A群,占比25%（n=5）；未检测到B1群菌株。通过MLST分型分析，20株大肠杆菌涉及10个ST型，其中ST131、ST648、ST744、ST1193型别较多。CRISPR序列扩增结果显示其中6株细菌含有CRISPR序列，提示不同菌株的进化差异。本研究通过对临床多重耐药UPEC进行毒力和分型特征分析，为临床有效防控耐药菌导致的尿路感染提供重要依据。 |
| 英文摘要: |
| This study investigated the drug resistance, virulence gene distribution and evolutionary typing characteristics of pathogens in clinical cases of urinary tract infection, so as to provide reference for the prevention and control of drug-resistant urinary tract infection pathogens. The collected urine samples were isolated and cultured, and the bacteria were identified by Gram staining and 16S rDNA. The bacteria were tested for drug sensitivity by K-B disk diffusion method, and the virulence genes and phylogenetic and evolutionary typing were analyzed by PCR amplification. In the study, 20 strains of uropathogenic Escherichia coli (UPEC) were successfully isolated. The drug sensitivity test showed that most strains had the characteristics of multiple drug resistance. The drug resistance rates of this batch of UPEC to penicillin, erythromycin and vancomycin reached 100%, more than 90% to carbenicillin, ampicillin and azithromycin, more than 80% to tetracycline, norfloxacin, streptomycin and streptomycin, more than 70% to ciprofloxacin, gentamicin, kanamycin, floxacin and ceftriaxone, more than 35% to cefotaxime, nitrofurantoin and chloramphenicol; The resistance rates to amoxicillin and polymyxin B were low, only 10% and 5% respectively. The detection rates of six virulence genes (iutA, fimH, hly, fyuA, tsh, traT) were 85%, 95%, 85%, 75%, 25% and 10% respectively. In its phylogenetic typing, the bacteria belonging to group B2 are the most, accounting for 40% (n=8), group D takes the second place, accounting for 35% (n=7), the third is group A, accounting for 25% (n=5); Group B1 strains weren't detected. Through MLST typing analysis, 20 strains of E. coli involved 10 ST types, including ST131, ST648, ST744, ST1193. CRISPR sequence amplification results showed that 6 strains of bacteria contained CRISPR sequence, suggesting the evolutionary differences of different strains. This study analyzed the virulence and typing characteristics of clinical multidrug-resistant UPEC, which provided a significant basis for clinical effective prevention and control of urinary tract infection caused by drug-resistant bacteria. |
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