| 柳立新,单锋,季婷婷,蒋长顺.大别山三个主产区野生及栽培天麻的8个主要成分含量比较[J].井冈山大学自然版,2022,43(3):21-26 |
| 大别山三个主产区野生及栽培天麻的8个主要成分含量比较 |
| COMPARISON OF EIGHT MAIN COMPONENTS BETWEEN THE WILD AND THE CULTIVATED GASTRODIA ELATA IN THREE MAIN PRODUCTION AREAS OF DABIE MOUNTAIN |
| 投稿时间：2021-12-29 修订日期：2022-01-26 |
| DOI：10.3969/j.issn.1674-8085.2022.03.004 |
| 中文关键词: 大别山区 天麻 含量比较 品质评价 |
| 英文关键词: Dabie Mountain area Gastrodia elata Bl. content comparison quality analysis |
| 基金项目:安徽省高校自然科学研究重点项目(KJ2018A0811,KJ2018A0804) |
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| 中文摘要: |
| 为评价安徽大别山区不同主产地天麻野生品及栽培品的品质优劣，选择8个主要指标成分，对三个主产区20个样品进行含量测定。采用Phenomenex Luna C18（250 mm×4.6 mm，5 μm）色谱柱，以0.1%甲酸乙腈（A）-0.1%甲酸水溶液（B）系统进行梯度洗脱。方法学显示天麻中腺苷、天麻素、对羟基苯甲醇、对羟基苯甲醛、香荚兰醇、巴利森苷C、巴利森苷B、巴利森苷A在相应检测范围内线性关系良好，r>0.9995，回收率（n=6）在97.21%~98.74%之间，相对偏差的RSD在0.14%~1.20%之间；并采用此方法对20个样品的天麻进行含量检测。结果显示本研究所采集的20个天麻样品，同一产地天麻的各指标差异较大，野生品有部分产地的含量相对较高，但与栽培品之间各指标的差异性不明显，提示大别山地区的三个天麻主产地的规范化种植取得较好的效果，后期通过种质培育和生境筛选的方式，对不同地区的天麻进行甄别，建立完善的、高质量的天麻GAP种植基地。 |
| 英文摘要: |
| To evaluate the quality of Gastrodia elata from different producing areas, we compared the contents of 8 main components of the wild and the cultivated G. elata in three main production areas of Dabie Mountain. 20 samples of G. elata from three main production areas were selected for the content determination. Phenomenex Luna C18 (250mm×4.6mm, 5 μm) was used as the chromatographic column, 0.1% formic acid acetonitrile (a) - 0.1% formic acid aqueous solution (b) was used as the moving phase with the gradient elution. It suggested that the linearity of adenosine, gastrodin, p-hydroxybenzyl alcohol, p-hydroxybenzaldehyde, vanilla alcohol, balisenside C, balisenside B and balisenside a in G. elata was good within the corresponding detection range (R > 0.9995), the recovery (n = 6) was 97.21% ~ 98.74%, and the RSD of relative deviation was 0.14% ~ 1.20%. This method was used to detect the contents of 20 varieties of G. elata. The results showed that among the 20 G. elata samples, the indexes from the same origin were quite different, and the contents of some wild products were relatively higher, but the differences of indexes between the wild and the cultivated products were not obvious, suggesting that the standardized planting of germplasm quality in the three main G. elata producing areas in Dabie Mountain had achieved good results. In the later stage, G. elata in different areas can be screened by germplasm cultivation and habitat screening to establish a perfect and high-quality GAP planting base of G. elata. |
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