| 乔晓燕,吴燕,高青海.氮素形态及配比对栝楼幼苗生长和氮代谢的影响[J].井冈山大学自然版,2021,42(6):46-51 |
| 氮素形态及配比对栝楼幼苗生长和氮代谢的影响 |
| EFFECT OF NITROGEN FORMS AND RATIOS ON THE GROWTH AND NITROGEN METABOLISM OF TRICHOSANTHES KIRILOWII SEEDLINGS |
| 投稿时间：2021-01-23 修订日期：2021-02-21 |
| DOI：10.3669/j.issn.1674-8085.2021.06.009 |
| 中文关键词: 氮素形态 栝楼 氮代谢 硝酸还原酶 |
| 英文关键词: nitrogen forms trichosanthes kirilowii nitrogen metabolism nitrate reductase |
| 基金项目:安徽高校自然科学研究重点项目(KJ2020A0079) |
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| 中文摘要: |
| 为探明氮素形态及配比对不同品种栝楼幼苗氮代谢生理特性的影响，本研究采用盆栽方法，以‘W3’和‘JX’两种栝楼幼苗为试验材料，设置6种硝铵比的氮素营养处理，测定栝楼幼苗生长量、根系活力、氮代谢关键酶活力、氨基酸含量和可溶性蛋白含量等。结果显示，与对照相比，硝态氮和铵态氮混合处理可提高栝楼幼苗氮代谢水平，提高栝楼氨基酸和可溶性蛋白含量，硝铵态氮配比为50：50处理‘W3’品种表现较好，其地上部和根系鲜重分别为45.4、5.68 g·plant-1，氮代谢酶GS、GOGAT、GDH活性最高；而‘JX’品种，硝铵态氮配比为70：30处理表现较好，植株鲜重和根系活力，氮代谢酶活性、氨基酸和可溶性蛋白含量高于其它处理。总之，硝态氮和铵态氮混合使用有利于栝楼幼苗生长，不同品种氮素比例有所不同。 |
| 英文摘要: |
| To investigate the effect of nitrogen forms and ratios on the physiological characteristics of nitrogen metabolism of the seedlings of Trichosanthes kirilowii among different varieties,‘W3’ and ‘JX’ seedlings were cultured under six nitrate and ammonium ratios in the pot, the plant growth, the activity of key enzymes in nitrogen metabolism, the content of amino acids and soluble proteins were detected. The results showed that, compared with the control group, the nitrogen metabolism, amino acid and soluble protein content of seedlings were increased by a mixture of NO3--N/NH4+——N ratio of 50:50. However, the treatment of ‘JX’ with NO3--N/NH4+——N ratio of 70:30 had a better performance, and the plant fresh weight, root activity, enzyme activity of nitrogen metabolism, amino acid and soluble protein content were higher than those of other treatments. In short, a mixture of nitrate and ammonium nitrogen favored the growth of seedlings of T. kirilowii, and the proportion of nitrogen in different varieties varied. |
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