| 张华,于虹敏,严国鸿,孙承韬.蛇伤胶囊中5种蒽醌类化合物含量的测定[J].井冈山大学自然版,2021,42(5):98-101 |
| 蛇伤胶囊中5种蒽醌类化合物含量的测定 |
| DETERMINATION OF FIVE ANTHRAQUINONES IN SHESHANG CAPSULES |
| 投稿时间：2021-02-16 修订日期：2021-05-19 |
| DOI：10.3669/j.issn.1674-8085.2021.05.019 |
| 中文关键词: 高效液相色谱法 蛇伤胶囊 蒽醌类 含量测定 |
| 英文关键词: HPLC Sheshang capsules anthraquinones quantitative determination |
| 基金项目:福建省科技厅科技引导性项目（2017Y0047） |
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| 摘要点击次数: 1569 |
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| 中文摘要: |
| 目的 建立蛇伤胶囊中5种蒽醌类成分含量测定分析方法，提升蛇伤胶囊质量标准。方法 采用Diamonsil C18色谱柱（250 mm×4.6 mm，5 μm），流动相为甲醇（A）-0.1%磷酸水溶液（B），梯度洗脱（0~7 min，75% A；7~30 min，75% A→90% A；30~40 min，90% A），流速为1.0 mL·min-1，检测波长为254 nm，进样量10 μL。结果 5个待测组的线性范围：4.04~40.40μg/mL（r=0.9994）、4.16~41.60μg/mL（r=0.9991）、4.14~41.40 μg/mL（r=0.9959）、4.10~41.00 μg/mL（r=0.9993）和2.16~21.60μg/mL（r=0.9997）。平均回收率（n=6）分别为100.02%、100.02%、100.03%、99.99%和100.06%，RSD分别为0.40%、0.15%、0.30%、0.11%和0.47%。结论 所建立的方法操作简便准确且具有良好的重复性，可用于测定蛇伤胶囊中芦荟大黄素、大黄素、大黄酸、大黄酚和大黄素甲醚的含量。 |
| 英文摘要: |
| Objective: To establish a quantitative determination method of HPLC for five anthraquinones and improve the quality control standard of Sheshang capsules. Methods: The separation was performed on a Diamonsil-C18 column (250 mm×4.6 mm, 5μm) with a gradient elution (0-7min, 75%A; 7-30 min, 75%A→90%A; 30~40 min, 90%A) of the mobile phase composed of methanol (A) and 0.1% phosphoric acid (B). The flow rate was 1.0 mL.min-1 and the detection wavelength was 254 nm. The injection volume was 10 μL. Results: The linear ranges of five anthraquinones were 4.04~40.40 μg/mL (r=0.9994), 4.16~41.60 μg/mL (r=0.9991), 4.14~41.40 μg/mL (r=0.9959), 4.10~41.00 μg/mL (r=0.9993) and 2.16~21.60 μg/mL (r=0.9997), respectively, and their average recoveries respectively were 100.02%, 100.02%, 100.03%, 99.99% and 100.06%, RSD respectively were 0.40%, 0.15%, 0.30%, 0.11% and 0.47% (n=6). Conclusion: The established method is simple, accurate and reproducible, can be used to determine the contents of aloe emodin, emodin, rhubarb, rhubarb phenol and emodin methy in Sheshang capsules. |
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