| 傅贵平,龚志南,王霞,李升,戴彩华,王冬芽,江嘉陵,武蓉,陈晨.升清降浊方对慢性肾功能衰竭重要脏器的保护效用及其对炎症因子的影响[J].井冈山大学自然版,2020,41(6):87-91 |
| 升清降浊方对慢性肾功能衰竭重要脏器的保护效用及其对炎症因子的影响 |
| PROTECTIVE EFFECT OF ASCENDING QING AND DESCENDING ZHUO PRESCRIPTION ON VITAL ORGANS IN CRF AND ITS INFLUENCE ON INFLAMMATORY FACTORS |
| 投稿时间：2020-07-16 修订日期：2020-09-16 |
| DOI：10.3969/j.issn.1674-8085.2020.06.017 |
| 中文关键词: 升清降浊方 CRF 炎症 转化生长因子 |
| 英文关键词: ascending qing and descending zhuo prescription CRF inflammation TGF-β |
| 基金项目:国家自然科学基金项目(81660784) |
| 作者 | 单位 | | 傅贵平 | 井冈山大学医学部, 江西, 吉安 343000 | | 龚志南 | 井冈山大学医学部, 江西, 吉安 343000 | | 王霞 | 井冈山大学医学部, 江西, 吉安 343000 | | 李升 | 井冈山大学医学部, 江西, 吉安 343000 | | 戴彩华 | 井冈山大学医学部, 江西, 吉安 343000 | | 王冬芽 | 井冈山大学医学部, 江西, 吉安 343000 | | 江嘉陵 | 井冈山大学医学部, 江西, 吉安 343000 | | 武蓉 | 井冈山大学医学部, 江西, 吉安 343000 | | 陈晨 | 井冈山大学医学部, 江西, 吉安 343000 |
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| 中文摘要: |
| 目的 探讨升清降浊方在延缓慢性肾功能衰竭(CRF) 发生发展过程中对重要脏器的保护效用及可能机制。方法 180 只SPF 级 SD 雄性大鼠被随机分为升清降浊方组、升麻单体治疗组、大黄单体治疗组、基本方治疗组、替米沙坦组和空白组,每组 30 只。采用大鼠 5/6 肾切除造模方法制备CRF模型,除空白组不给药物干预外,其余各组连续给药12周。灌药前后采集大鼠血液,检测Scr、BUN、UA等肾功能指标以及C 反应蛋白(CRP)、白细胞介素 1 (IL-1)、白细胞介素 6 (IL-6)肿瘤坏死因子 α (TNF-α) 等炎症因子指标的变化;取大鼠肾脏、心脏和肝脏组织观察不同分组各组织的病理改变。结果 升清降浊方组、升麻单体治疗组、大黄单体治疗组、基本方治疗组、替米沙坦组大鼠Scr、BUN、UA、CRP、IL-1、IL-6、TNF-α 较空白组有明显差异;升清降浊方组、升麻单体治疗组、大黄单体治疗组治疗前后Scr、BUN、UA、CRP、IL-1、IL-6、TNF-α有显著差异;升清降浊方组的Scr、UA、IL-1、IL-6、TNF-α较其它治疗组降低更显著。病理切片结果显示升清降浊方组对大鼠肾脏、心脏及肝脏保护明显优于其他组。结论 升清降浊方可能通过调控炎症因子的表达以达到阻断CRF进展,改善重要脏器功能。 |
| 英文摘要: |
| Objective: To investigate the protective effects and potential mechanism of ascending qing and descending zhuo prescription in the process of delaying the occurrence and development of CRF. Methods: 180 SPF SD male rats were randomly divided into ascending qing and descending zhuo prescription group, rhizoma cimicifugae monotherapy group, rhubarb monotherapy group, basic formula group, telmisartan group and blank group, with 30 rats in each group. 5/6 nephrectomy of rats could be used for establishing CRF model. Except for the blank group, the other groups were given drugs for 12 weeks. Before and after intragastric administration, blood samples of rats were collected to detect the changes of renal function indicators (SCR, bun and UA) and the inflammatory indexes (CRP, IL-1, IL-6 and TNF-α); the renal, heart and liver tissues of rats were observed to analyze pathological changes in different groups. Results: There were significant differences in Scr, BUN, UA, CRP, IL-1, IL-6 and TNF-α of the rats from ascending qing and descending zhuo prescription group, rhizoma cimicifugae monotherapy group, rhubarb monotherapy group, basic formula group and telmisartan group, compared with that of blank group; Scr, BUN, UA, CRP, IL-1, IL-6 and TNF-α of ascending qing and descending zhuo prescription group, rhizoma cimicifugae monotherapy group and rhubarb monotherapy group were notably different before and after treatment; SCR, UA, IL-1, IL-6 and TNF–α in ascending qing and descending zhuo prescription group were significantly lower than those in other groups. The pathological section showed that the protection of kidney, heart and liver in ascending qing and descending zhuo prescription group was better than that in other groups. Conclusion: Ascending qing and descending zhuo prescription could regulate the expression of inflammatory factors to block the progress of CRF and improve vital organ functions. |
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