| 李彩虹,李银蕊,胡赞,母育成,李春艳,陈俊雅.云南松松塔对肺纤维化大鼠炎症因子的作用[J].井冈山大学自然版,2020,41(5):92-97 |
| 云南松松塔对肺纤维化大鼠炎症因子的作用 |
| EFFECT OF THE PINECONE OF PINUS YUNNANENSIS ON INFLAMMATORY FACTORS IN RATS WITH PULMONARY FIBROSIS |
| 投稿时间：2020-02-26 修订日期：2020-04-17 |
| DOI：10.3969/j.issn.1674-8085.2020.05.016 |
| 中文关键词: 肺纤维化 云南松松塔 转化生长因子β1 肿瘤坏死因子α 羟脯氨酸 |
| 英文关键词: pulmonary fibrosis pinecone of Pinus yunnanensis transform growth factor β1 tumor necrosis factor α hydroxyproline |
| 基金项目:云南省科技厅应用基础研究青年项目（2017FD137）；大理大学大学生科研基金项目（KYSX201682） |
| 作者 | 单位 | | 李彩虹 | 大理大学药学与化学学院, 云南, 大理 671000 | | 李银蕊 | 大理大学药学与化学学院, 云南, 大理 671000 | | 胡赞 | 大理大学药学与化学学院, 云南, 大理 671000 | | 母育成 | 大理大学药学与化学学院, 云南, 大理 671000 | | 李春艳 | 大理大学药学与化学学院, 云南, 大理 671000 | | 陈俊雅 | 大理大学药学与化学学院, 云南, 大理 671000 |
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| 中文摘要: |
| 目的 探讨云南松松塔提取物（Extracts from the pinecone of Pinus yunnanensis，PE）对肺纤维化大鼠血清炎症因子水平的影响。方法 SD大鼠随机分为假手术组、模型组、阳性组（5 mg/kg醋酸泼尼松龙）及PE高、中、低剂量组（200、100、50 mg/kg）。采用气管内一次性注射0.2 mL博来霉素生理盐水溶液（5 mg/kg）建立大鼠肺纤维化模型，假手术组气管内给予0.2 mL无菌生理盐水。造模后第2 d开始给予相应药物，分别于实验第7、14、28 d，处死1/3的大鼠，测定大鼠肺系数，酶联免疫吸附试验（Enzyme linked immunosorbent assay，ELISA）测定血清中羟脯氨酸（Hydroxyproline，HYP）、转化生长因子beta 1（Transform growth factor beta 1，TGF-β1）、肿瘤坏死因子alpha（Tumor necrosis factor alpha，TNF-α）的含量，苏木素-伊红（Hematoxylin-eosin，HE）染色考察肺脏组织病理学变化。结果 与假手术组比较，模型组大鼠在第7、14、28 d肺系数、血清HYP、TGF-β1、TNF-α含量均明显升高（P<0.05，P<0.01）；与模型组比较，PE中、高剂量组可以不同程度的减小肺系数（P<0.05，P<0.01），降低血清中TGF-β1、TNF-α及HYP含量（P<0.05，P<0.01）。HE染色显示PE中、高剂量组大鼠肺泡结构损伤及炎性细胞浸润均较模型组明显减轻。结论 云南松松塔可以改善博来霉素诱导肺纤维化大鼠的炎症反应和胶原沉积情况。 |
| 英文摘要: |
| Objective: To study the effects of the extracts from pinecone of Pinus yunnanensis on inflammatory factors of rats with pulmonary fibrosis. Methods: SD rats were randomly divided into sham-operated group, model group, positive group (5 mg/kg prednisolone) and PE high, middle and low dosage groups (200, 100, 50 mg/kg). The pulmonary fibrosis model was established by intratracheally administration 0.2 mL bleomycin (5 mg/kg), and the rats in sham-operated group were given 0.2 mL normal saline. The corresponding drugs were administrated to the rats from the 2nd day, and then the 1/3 rats in each group were sacrificed to the 7th, 14th and 28th d, respectively. Finally, the lung index was determined. The contents of HYP, TGF-β1, TNF-α in serum were determined through ELISA, and histopathological change was investigated by HE staining. Results: The lung index, contents of HYP, TGF-β1, TNF-α in serum of rats in model group were significantly increased (P<0.05, P<0.01) compared with sham-operated group. Compared with the model group, the lung index, the contents of TGF-β1, TNF-α and HYP in PE high and middle dose groups decreased apparently (P<0.05, P<0.01). Histopathological sections showed damaged alveolar structure and inflammatory cells infiltration were alleviated in PE middle and high groups compared with the model group. Conclusion: The pinecone of P. yunnanensis could ameliorate lung fibrosis, inflammatory response and collagen deposition in rats with pulmonary fibrosis induced by bleomycin. |
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