| 唐梅,杨胜祥,赵立春.青钱柳叶降血糖作用研究[J].井冈山大学自然版,2020,41(5):83-91 |
| 青钱柳叶降血糖作用研究 |
| HYPOGLYCEMIC EFFECT OF EXTRACTS FROM CYCLOCARYA PALIURUS (BATAI.) ILJINSKAJA LEAVES |
| 投稿时间：2020-02-12 修订日期：2020-04-27 |
| DOI：10.3969/j.issn.1674-8085.2020.05.015 |
| 中文关键词: 青钱柳叶 双水相提取 降血糖 |
| 英文关键词: Cyclocarya paliurus (Batai.) Iljinskaja leaves aqueous two-phase extracts hypoglycemic effect |
| 基金项目:广西一流学科项目（05019018A5）;“岐黄工程”创新研究团队项目（2018002）；广西中医药大学2018年自治区级硕士研究生科研创新项目（YCSW2018169） |
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| 中文摘要: |
| 目的 研究青钱柳叶双水相提取物对糖尿病小鼠的降血糖作用。方法 通过高脂饮食联合腹腔注射链佐霉素（STZ）建立小鼠糖尿病模型，设置青钱柳叶双水相提取物高、中、低剂量组灌胃，连续14 d后测定空腹血糖值，检测血清中胰岛素（INS）、总胆固醇（T-CHO）、甘油三酯（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）、极低密度脂蛋白含量（VLDL-C），采用HE染色法观察实验性糖尿病小鼠肝脏和胰腺的病理学变化。结果 青钱柳叶双水相提取物中剂量能明显改善2型糖尿病小鼠体重减轻这一症状（P<0.01），低剂量组降低糖尿病小鼠空腹血糖（P<0.05），高剂量组显示出降低血清胰岛素的作用（P<0.05），青钱柳叶双水相提取物能降低糖尿病小鼠血清中总胆固醇、甘油三酯、低密度脂蛋白的含量，增加高密度脂蛋白含量。二甲双胍组和青钱柳叶双水相提取物高、中、低剂量组小鼠的肝脏和胰腺的病变较模型组有不同程度的减轻。结论 青钱柳叶双水相提取物具有降低小鼠空腹血糖、降脂、改善胰岛素抵抗、保护胰岛细胞的作用，其降血糖的作用机制需进一步研究。 |
| 英文摘要: |
| Objective: The effects of aqueous two-phase extracts of Cyclocarya paliurus (Batai.) Iljinskaja leaves on diabetes mellitus mice were studied. Methods: The mice were fed with high fat diet, then intraperitoneal injected streptozotocin (STZ) to establish the T2DM animal models. After two weeks, the contents of insulin (INS), total cholesterol (T-CHO), serum triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C) and very low density lipoprotein (VLDL-C) were measured. The changes of liver and pancreatic tissues pathology in experimental diabetic mice were observed by the immunohistochemical method. Results: Middle dose group of C. paliurus leaves aqueous two-phase extracts could significantly improve the loss of diabetic mice weights (P<0.01). Low dose group lowered the fasting blood glucose and high dose group lowered INS (P<0.05). C. paliurus leaves aqueous two-phase extracts could reduce the contents of T-CHO, TG, LDL-C and increased HDL-C. T2DM mice hepatocytes showed different degrees of edema and degeneration, the portal areas and lobule occurred inflammatory cell infiltration. The liver changes of mice administrated by C. paliurus leaves extracts and metformin hydrochloride alleviated compared with the model group. Compared with normal mice, there were fewer islets, irregular morphology, uneven cell distribution in the islets of T2DM mice. Conclusion: C. paliurus leaves aqueous two-phase extracts possessed lowering fasting blood glucose, lowering lipid, improving insulin resistance and protective effects on STZ diabetic mice, while its specific mechanisms were worth for further investigation. |
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