| 郑卓,江振方,王复标,孙惠敏.水稻早熟大穗品系7-37二次枝梗数的QTL定位研究[J].井冈山大学自然版,2020,41(5):31-37 |
| 水稻早熟大穗品系7-37二次枝梗数的QTL定位研究 |
| QTL MAPPING OF SECONDARY PANICLE BRANCH NUMBER OF A PREMATURE AND LARGE-EAR VARIETY 7-37 IN RICE |
| 投稿时间：2020-03-06 修订日期：2020-05-09 |
| DOI：10.3969/j.issn.1674-8085.2020.05.007 |
| 中文关键词: 水稻 二次枝梗数 QTL定位 |
| 英文关键词: rice secondary panicle branch number QTL mapping |
| 基金项目:国家自然科学基金项目（31660382） |
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| 摘要点击次数: 1660 |
| 全文下载次数: 1947 |
| 中文摘要: |
| 通过水稻穗部性状的改良尤其是增加二次枝梗数更有助于实现水稻的大穗和高产。本研究以7-37作为二次枝梗数QTL定位的亲本材料，通过小穗广亲和品种02428与7-37杂交并自交获得02428/7-37F2，然后对该F2群体中随机选取的284个单株主茎穗的二次枝梗数进行遗传分析，结果表明二次枝梗数呈典型的正态分布，说明二次枝梗数受多基因控制，属数量性状，与前人的研究结果是一致的；在此基础上，利用从339对SSR引物中筛选出的均匀分布于12条染色体上的58对多态性引物对F2群体中的284个单株进行PCR扩增分析，再利用QTL Icimapping作图软件，采用复合区间作图法对7-37的二次枝梗数进行了QTL分析和作图，结果在4号染色体25.0~30.5 Mb区间内和6号染色体6.6~30.8Mb区间内分别定位出了二次枝梗数相关QTL位点，LOD值分别为5.73和2.56，贡献率分别为8.95%和4.38%，加性效应分别为-5.71和-3.85，显性效应分别为-2.83和-1.30。这些研究结果为下一步二次枝梗数QTL位点的精细定位、克隆和功能分析提供了研究基础。 |
| 英文摘要: |
| The improvement of panicle traits, especially the increase of the numbers of secondary branches, is more conducive to the realization of the large ear and high yield of rice. In this study, 7-37 was used as the parental material for QTL mapping of secondary panicle branch number. The hybrid between the variety 02428 and 7-37 was carried out by spikelet broad-affinity, and 02428/7-37F2 was obtained by self-crossing of the hybrid 02428/7-37F1. The numbers of secondary panicle branches of each main stem panicle selected from the F2 population randomly were genetically analyzed. The results showed that the numbers of secondary panicle branches were normally distributed, indicating that the number of secondary branches was controlled by multiple genes and belonged to quantitative traits. On this basis, PCR was performed on the 284 individual plants selected from the F2 population randomly by the 58 pairs of the SSR primers which were uniformly distributed on 12 chromosomes, and QTL analysis and mapping were performed on the secondary panicle branches number of 7-37 using the QTL Icimapping software. The results showed that the QTL locus associated with the secondary panicle branche number are located in the 25.0-30.5 Mb range of chromosome 4 and the 6.6-30.8Mb range of chromosome 6, the LOD values are 5.73 and 2.56, the contribution rates are 8.95% and 4.38%, the additive effects were -5.71 and -3.85, and the dominant effects are -2.83 and -1.30 respectively. These results provide a basis for the fine mapping, cloning and functional analysis of the QTL locus of the secondary panicle branch number in the future. |
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