| 姚武,罗辉.HPLC法检测艾叶中伞形花内酯含量[J].井冈山大学自然版,2020,41(1):21-24 |
| HPLC法检测艾叶中伞形花内酯含量 |
| DETERMINATION OF UMBELLIFERONE IN FOLIUM ARTEMISIAE ARGYI BY HPLC |
| 投稿时间：2019-12-26 修订日期：2019-12-26 |
| DOI：10.3969/j.issn.1674-8085.2020.01.005 |
| 中文关键词: 艾叶 伞形花内酯 高效液相色谱 检测 |
| 英文关键词: folium artemisiae argyi umbelliferone HPLC determination |
| 基金项目:国家自然科学基金项目（31460488）；安徽省自然科学基金面上项目（11040606M41）；安徽省高校自然科学重点研究项目（KJ2016A683） |
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| 中文摘要: |
| 本实验首次运用HPLC方法分析了艾叶中伞形花内酯的含量。采用Agilent TC-C18色谱柱（250 mm×4.6 mm，5 μm），甲醇-水-四氢呋喃（4：15：1）为流动相，流速为1.0 mL/min，检测波长为325 nm。实验结果显示，伞形花内酯在0.1~2.0 μg/mL浓度范围内与峰面积之间具有良好的线性关系，回归方程为A=74.3869C-0.6815（R=0.9998），平均回收率为99.5%，RSD为1.06%。本法操作简便、高效、准确，可以为建立和完善中药材艾叶的质量标准提供了借鉴。 |
| 英文摘要: |
| In this paper, the content of umbelliferone in folium artemisiae argyi was firstly detected by HPLC method. Agilent TC-C18 column (250 mm×4.6 mm, 5 μm) was used with a methanol-water-tetrahydrofuran (4:15:1) solution as the mobile phase at a flow rate of 1.0 mL/min. The UV detection wavelength was 325 nm. The concentration of umbelliferone showed good linear relationship with the peak area in the range of 0.1~2.0 μg/mL. The regression equation was A=74.3869C-0.6815 (R=0.9998). The average recovery rate was 99.5% (RSD=1.06%). This method is simple, rapid and accurate, and provides a reference for the establishment and improvement of the quality standard of folium artemisiae argyi. |
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