井冈山大学学报自然科学版

文章摘要

陈静,张凤,张锐,李卿,范惠珍.LC-MS/MS法测定毛曼陀罗中莨菪碱、东莨菪碱和山莨菪碱的含量[J].井冈山大学自然版,2019,40(4):92-95

LC-MS/MS法测定毛曼陀罗中莨菪碱、东莨菪碱和山莨菪碱的含量

DETERMINATION OF HYOSCYAMINE, SCOPOLAMINE AND ANISODAMINE IN DATURA INNOXIA BY LC-MS/MS

投稿时间：2019-01-09 修订日期：2019-04-17

DOI：10.3969/j.issn.1674-8085.2019.04.018

中文关键词: 莨菪碱东莨菪碱山莨菪碱曼陀罗

英文关键词: hyoscyamine scopolamine anisodamine Datura innoxia

基金项目:国家自然科学基金项目（31770329，81303160）

作者	单位
陈静	宜春学院化学与生物工程学院, 江西, 宜春 336000
张凤	第二军医大学长征医院药材科, 上海 20003
张锐	宜春学院化学与生物工程学院, 江西, 宜春 336000
李卿	第二军医大学长征医院药材科, 上海 20003
范惠珍	宜春学院化学与生物工程学院, 江西, 宜春 336000

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中文摘要:

目的建立LC-MS/MS测定毛曼陀罗中莨菪碱、东莨菪碱和山莨菪碱含量的方法。方法利用盐酸与浓氨水和三氯甲烷提取毛曼陀罗根部中的生物碱，以乙腈-（0.05%甲酸）水溶液=68：32为流动相；Agilent ZORBAXSB-C18（2.1 mm×100 mm，3.5 μm）的色谱柱；柱温25℃；流速0.3 mL/min；进样量：2 μL。结果莨菪碱、东莨菪碱和山莨菪碱在分别为1~200 ng/mL；1~1000 ng/mL；1~1000 ng/mL的范围内有良好的线性关系。线性方程依次为：Y=813.8904X+2165.3000（r²=0.9922）；Y=714.1221X+517.4311（r²=0.9892）；Y=872.9971X+1024.4247（r²=0.9952）；平均加样回收率分别为97.17%、97.2%、92.68%，RSD分别为1.02%、1.31%、1.41%。通过对12批曼陀罗根中莨菪碱、东莨菪碱和山莨菪碱的测定，其浓度范围分别为1~20 ng/mL；1~18 ng/mL；1~19 ng/mL。结论该方法简单、灵敏度高、稳定性好可用于毛曼陀罗中莨菪碱、东莨菪碱和山莨菪碱含量的测定。

英文摘要:

Objective:To establish a LC-MS/MS method for the determination of hyoscyamine, scopolamine and anisodamine in Datura innoxia. Methods:The alkaloids were extracted with hydrochloric acid, ammonium hydroxide and chloroform. The chromatographic column was Agilent ZORBAX SB-C18 (2.1×100 mm, 3.5 μm), the moving phase was composed of acetonitrile and 0.05% formic acid solution with the ratio of 68:32, the column temperature was 25℃, the flow rate was 0.3 mL/min and the injection amount was 2 μL. Results:The linear ranges of hyoscyamine, scopolamine and anisodamine were 1 ng/mL~200 ng/mL, 1 ng/mL~1000 ng/mL and 1 ng/m~1000 ng/mL respectively. The linear equations were Y=813.8904X + 2165.3000 (R²=0.9922), Y=714.1221X + 517.4311 (R²=0.9892), Y=872.9971X + 1024.4247 (R²=0.9952) respectively. The average recoveries were 97.17%, 97.2%, 92.68%, and RSD were 1.02%, 1.31% and 1.41% respectively. Conclusion:The method is simple, sensitive and stable for the determination of hyoscyamine, scopolamine and anisodamine.

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