| 赖昕,林珠灿,余丽双,黄雪雪,陈莉.消癥益肝片中抗肝癌活性成分的分离纯化和结构鉴定[J].井冈山大学自然版,2018,(2):93-96 |
| 消癥益肝片中抗肝癌活性成分的分离纯化和结构鉴定 |
| ISOLATION, PURIFICATION AND STRUCTURAL IDENTIFICATION OF ANTI-HEPATOMA COMPONENTS FROM XIAOZHENGYIGAN TABLETS |
| 投稿时间：2017-12-26 修订日期：2018-01-26 |
| DOI：10.3969/j.issn.1674-8085.2018.02.017 |
| 中文关键词: 消癥益肝片 分离纯化 结构鉴定 抗肝癌 |
| 英文关键词: Xiaozhengyigan tablets isolation and purification structure identification anti-hepatoma |
| 基金项目:福建省科技厅社发处引导性项目（2016Y0054，2017Y0051）；福建省教育厅科技项目（JZ160445）；福建省教育厅中青年教师教育科研项目（JAT160245）；福建中医药大学校管课题（X2015012-平台，X2015010-平台） |
| 作者 | 单位 | | 赖昕 | 福建中医药大学药学院, 福建, 福州 350122 | | 林珠灿 | 福建中医药大学药学院, 福建, 福州 350122 | | 余丽双 | 福建中医药大学药学院, 福建, 福州 350122 | | 黄雪雪 | 福建中医药大学药学院, 福建, 福州 350122 | | 陈莉 | 福建中医药大学药学院, 福建, 福州 350122 |
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| 中文摘要: |
| 目的 分离、纯化及鉴定消癥益肝片中抗肝癌的活性物质。方法 采用Sephadex LH20和反相制备高效液相色谱柱分离纯化；采用MS、1H-NMR等波谱分析进行结构鉴定；利用MTT法检测化合物的抗肝癌活性。结果 经分离纯化，获得3个活性小分子化合物，分别为苯甲酸（I）、对羟基苯甲酸乙酯（Ⅱ）、对羟基苯甲酸丙酯（Ⅲ）。MTT实验表明，三个化合物均有体外抑制HepG2细胞增殖的活性，其中化合物Ⅱ和Ⅲ作用较强，并呈时间-剂量依赖关系。结论 鉴定出3个小分子化合物，均为首次从消癥益肝片中分离得到，具有体外抑制肝癌细胞生长的活性。 |
| 英文摘要: |
| Objective:To isolate, purify and identify the anti-hepatoma constituents from Xiaozhengyigan tablets (XZYG). Method:Anti-hepatoma chemical constituents were isolated and purified by Sephadex LH-20 and preparative RP-HPLC. The chemical structures were identified by MS and 1H-NMR. The effect on the proliferation of human liver cancer cell line (HepG2) was evaluated by MTT assay. Results:Three chemical constituents were obtained and their structures were determined as benzoic acid (I), Ethyl p-hydroxybenzoate (Ⅱ), Propyl-p-hydroxybenzoate (Ⅲ), respectively, based on MS and 1H-NMR data and comparison with the literatures. All of the three compounds were isolated from XZYG for the first time and had growth inhibitory activity on HepG2 cells. The inhibitory effect of Ⅱ and Ⅲ on HepG2 cells showed a concentration-dependent manner. Conclusion:Three small molecules were isolated from XZYG which may inhibit tumor cell growth in vitro. |
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