| 孙莉娜,林常青,郑志勇.催化H2O2氧化吖啶黄固体基质室温磷光法测定痕量秋水仙碱[J].井冈山大学自然版,2018,(2):23-30 |
| 催化H2O2氧化吖啶黄固体基质室温磷光法测定痕量秋水仙碱 |
| ULTRA-SENSITIVE SOLID SUBSTRATE-ROOM TEMPERATURE PHOSPHORIMETRY FOR COLCHICINE DETECTION BASED ON ITS CATALYTIC EFFECT ON H2O2 OXIDIZE ACRIDINE YELLOW |
| 投稿时间：2017-12-26 修订日期：2018-02-16 |
| DOI：10.3969/j.issn.1674-8085.2018.02.005 |
| 中文关键词: 秋水仙碱 吖啶黄 过氧化氢 磷光猝灭法 |
| 英文关键词: colchicines acridine yellow hydrogen peroxide phosphor quenching |
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| 中文摘要: |
| 基于秋水仙碱（COL）能催化过氧化氢（H2O2）氧化吖啶黄（AY）的反应，导致AY的室温磷光信号（RTP）剧烈猝灭，据此建立了超灵敏催化H2O2氧化AY固体基质室温磷光法（SS-RTP）测定COL的新方法。此方法的量化限（LOQ）为0.12 fg/斑（对应浓度为3.1×10-13 g·mL-1），灵敏度高、简便、快捷、准确。用于血清中COL的测定，结果与UPLC-MS/MS相吻合。同时测定了动力学常数，其活化能（E）为40.53 kJ·mol-1，速度反应常数（k）为3.97×10-4·s-1。 |
| 英文摘要: |
| A new solid substrate-room temperature phosphorimetry (SS-RTP) for colchicine (COL) detection has been established based on its strong catalytic effect on H2O2 oxidize acridine yellow(AY), which caused the room temperature phosphorimetry (RTP) of AY to quench sharply. This high sensitive (limit of quantification (LOQ):3.1×10-13 g·mL-1), accurate and selective SS-RTP has been successfully applied in the COL detection in the human serum and tea samples with the results agreeing well with high performance liquid chromatography (HPLC), the results were coincident with those of ultra fast high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. The activation energy and the reaction rate constant of catalytic reaction were 40.53 kJ·mol-1 and 3.97×10-4·s-1, respectively. |
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