| 曾常爱,孙小春.dentonin对牙髓干细胞增殖的实验研究[J].井冈山大学自然版,2017,(3):93-95 |
| dentonin对牙髓干细胞增殖的实验研究 |
| RESEARCH ON THE EFFECT OF DENTONIN FOR DENTAL PULP STEM CELLS PROLIFERATION |
| 投稿时间：2017-02-12 修订日期：2017-04-06 |
| DOI：10.3969/j.issn.1674-8085.2017.03.017 |
| 中文关键词: dentonin 牙髓干细胞 增殖 |
| 英文关键词: dentonin dental pulp stem cells proliferation |
| 基金项目:江西省科技厅科技计划项目（20122BBG70151-2） |
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| 中文摘要: |
| 目的 研究dentonin促进牙髓干细胞增殖，获得有关dentonin对牙髓干细胞增殖的实验证据。方法 把构建好的含有真核细胞表达质粒pcDNA-dentonin，在脂质体LipofectAMINE介导下，转染到体外培养的牙髓干细胞，再进行MTT检测细胞增殖。结果 发现dentonin对牙髓干细胞生长有明显的增殖作用。结论 牙髓干细胞生长速度较快，但是在两周后进入平台期，牙髓干细胞生长速度减慢。 |
| 英文摘要: |
| Objective: To study the promotion effect of dentonin on dental pulp stem cells proliferation and obtain the relative experimental evidence. Methods: Eukaryotic cells expression plasmid pcDNA-dentonin was transfected into dental pulp stem cells cultured in vitro by being mediated by LipofectAMINE, and then the cell proliferation were determined by MTT. Results: We found dentonin an obvious proliferation effect on dental pulp stem cells. Conclusion: The cell growth rate was faster, but cell growth had slowed in the plateau after two weeks. |
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