| 董晶,梁兆昌,陈海芳,宋玉鹏,杨武亮.HPLC法同时测定不同采收期江枳壳中辛弗林和N-甲基酪胺的含量[J].井冈山大学自然版,2016,(2):92-96 |
| HPLC法同时测定不同采收期江枳壳中辛弗林和N-甲基酪胺的含量 |
| SIMULTANEOUS DETERMINATION OF SYNEPHRINE AND N-METHYLTYRAMINE IN JIANGXI AURANTII FRUCTUS AT DIFFERENT HARVEST TIME BY HPLC |
| 投稿时间：2015-11-19 修订日期：2016-01-18 |
| DOI：10.3969/j.issn.1674-8085.2016.02.019 |
| 中文关键词: HPLC 含量测定 江枳壳 不同采收期 辛弗林 N-甲基酪胺 |
| 英文关键词: HPLC quantification Jiangxi aurantii fructus different harvest time synephrine N-methyltyramine |
| 基金项目:国家自然科学基金项目(81460575,81260612);江西省自然科学基金项目(20132BAB205092) |
| 作者 | 单位 | E-mail | | 董晶 | 江西中医药大学现代中药制剂教育部重点实验室, 江西, 南昌 330004 | | | 梁兆昌 | 井冈山大学医学院, 江西, 吉安 343009 | | | 陈海芳 | 江西中医药大学现代中药制剂教育部重点实验室, 江西, 南昌 330004 | | | 宋玉鹏 | 江西中医药大学现代中药制剂教育部重点实验室, 江西, 南昌 330004 | | | 杨武亮 | 江西中医药大学现代中药制剂教育部重点实验室, 江西, 南昌 330004 | yangwuliang@163.com |
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| 中文摘要: |
| 目的 采用HPLC法同时测定不同采收期江枳壳中辛弗林和N-甲基酪胺的含量,考察不同采收期江枳壳中上述两种生物碱含量的变化。方法 采用高效液相色谱法。结果 辛弗林与N-甲基酪胺分别在1.09~5.45 μg(R2 = 0.9997),0.18~0.90 μg(R2 = 0.9999)范围内呈良好的线性关系;其平均回收率(n= 6)分别为102.30%和98.07%,RSD分别为2.12%和2.20% ;江枳壳样品中辛弗林和N-甲基酪胺的含量分别在0.07%~0.43%和0%~0.06%的范围内。结论 辛弗林含量随采收期的推迟而降低,N-甲基酪胺在当年7月份以后检测不到含量。该方法简便、准确、灵敏度高,可用于辛弗林和N-甲基酪胺的含量测定。 |
| 英文摘要: |
| Objective: To determine the contents of synephrine and N-methyltyramine in Jiangxi aurantii fructus at different harvest time by HPLC, and to investigate the changes of the two components. Methods: HPLC was applied in the experiment. Results: The method had good linear relationship within the range of 1.09~5.45 μg(R2 =0.9997)for synephrine and 0.18~0.90 μg(R2 = 0.9999)for N-methyltyramine. The average recoveries ( n = 6) were 102.30% with RSD of 2.12% for synephrine and 98.07% with RSD of 2.20% for N-methyltyramine respectively. The contents in Jiangxi aurantii fructus were 0.07%~0.43% for synephrine and 0~0.06% for N-methyltyramine respectively. Conclusion: The content of synephrine were low at late harvest time, N-methyltyramine wasn't detected since July. The method is convenient, accurate and highly sensitive, and can be applied to the determination of synephrine and N-methyltyramine in aurantii fructus. |
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