| 黄春芳,陈华龙.一步法合成Fe3O4/PDA-PtNPs-Hb复合磁性纳米粒子及其对H2O2电催化性能的研究[J].井冈山大学自然版,2016,(2):24-28 |
| 一步法合成Fe3O4/PDA-PtNPs-Hb复合磁性纳米粒子及其对H2O2电催化性能的研究 |
| ONE-POT PREPARATION OF FE3O4/ITS PDA-PTNPS-HB COMPOSITE MAGNETIC NANOPARTICLES AND ITS ELECTROCATALYTIC ACTIVITIES ON H2O2 REDUCTION |
| 投稿时间：2015-12-02 修订日期：2016-02-02 |
| DOI：10.3969/j.issn.1674-8085.2016.02.006 |
| 中文关键词: 血红蛋白 复合磁性纳米粒子 直接电化学 生物传感器 |
| 英文关键词: hemoglobin composite magnetic nanoparticles direct electrochemistry biosensor |
| 基金项目:江西省自然科学基金项目(20132BA213010) |
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| 摘要点击次数: 2034 |
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| 中文摘要: |
| 以氯铂酸为氧化剂、Fe3O4纳米粒子为载体、血红蛋白(Hb)为模型蛋白,利用多巴胺(DA)氧化聚合生成聚多巴胺(PDA),同时氯铂酸还原为铂纳米粒子(PtNPs)的性质,一步法合成了Fe3O4/PDA-PtNPs-Hb复合磁性纳米粒子。将Fe3O4/PDA-PtNPs-Hb固定于磁性玻碳基底表面制得Fe3O4/PDA-PtNPs-Hb/MGC电极。对固定在Fe3O4/PDA-PtNPs-Hb复合磁性纳米粒子中的Hb在电极上的直接电化学行为进行了研究,结果表明Fe3O4/PDA-PtNPs-Hb复合磁性纳米粒子不仅能简便地固定在电极表面,而且能有效地促进Hb与电极表面的直接电子转移。此外,Fe3O4/PDA-PtNPs-Hb/MGC电极对H2O2有很好的电催化活性,在6.6 ~ 72.6 μM范围内具有良好的线性响应,检测限达3.92 μM(S/N=3)。 |
| 英文摘要: |
| Fe3O4/PDA-PtNPs-Hb composite magnetic nanoparticles were prepared in one-pot by employing hemoglobin (Hb) as model protein. In the presence of H2PtCl6, dopamine (DA) was oxidized forming polydopamine (PDA) on the surface of the Fe3O4 magnetic nanoparticles. Meanwhile, the formed Pt nanoparticles and Hb were embedded in the PDA. The Fe3O4/PDA-PtNPs-Hb/MGC electrode was conveniently prepared by simply immobilizing Fe3O4/PDA-PtNPs-Hb on the magnetic glassy carbon (MGC) electrode for H2O2 reduction. The results demonstrated that Fe3O4/PDA-PtNPs-Hb could maintain the bioactivity and facilitate the direct electrochemisty of Hb in the Fe3O4/PDA-PtNPs-Hb/MGC electrode. The formed Fe3O4/PDA-PtNPs-Hb/MGC electrode showed good electrocatalytic activity on the reduction of H2O2 and was used to detect H2O2 in the range of 6.6 ~ 72.6 μM with the detection of limit of 3.92 μM (S/N=3). |
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