| 汪洪,蔡进章,曾建军.重组人成纤维细胞生长因子21对胡萝卜遗传转化的研究[J].井冈山大学自然版,2016,(1):57-61 |
| 重组人成纤维细胞生长因子21对胡萝卜遗传转化的研究 |
| GENETIC TRANSFORMATION OF RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR 21 (hFGF21) ON DAUCUS CAROTA L. |
| 投稿时间：2015-10-07 修订日期：2015-12-17 |
| DOI：10.3969/j.issn.1674-8085.2016.01.012 |
| 中文关键词: FGF 21 胡萝卜 农杆菌 遗传转化 |
| 英文关键词: hFGF21 Daucus carota L. Agrobacterium tumefaciens genetic transformation |
| 基金项目:浙江省自然科学基金项目(Y3100459) |
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| 中文摘要: |
| 人成纤维细胞生长因子21 (FGF21) 是肝脏合成的一种可分泌多肽,对糖脂代谢的调控具有重要作用。以胡萝卜(Daucus carota L.) 七寸人参品种为试验材料,用含重组人成纤维细胞生长因子21(rhFGF21)质粒p1390 RⅡ的根癌农杆菌LBA4404对胡萝卜进行了遗传转化实验。PCR扩增和Western blot结果表明外源基因FGF21已成功转入胡萝卜基因组中。此研究为今后用胡萝卜产业化表达生产FGF21提供参考。 |
| 英文摘要: |
| Human fibroblast growth factor 21 (hFGF21), found in the mouse embryo, is a new member of fibroblast growth factor family. It is synthesized in the liver as secreting protein. In this study, Daucus carota L. hypocotyl was used as experimental materials. Recombinant human fibroblast growth factor 21 (rhFGF21) was introduced into D. carota via Agrobacterium tumefaciens-mediated method. The regenerated hygmycin resistant plantlets were confirmed by PCR and Western blotting. The results provided an experimental basis for large-scale production of FGF21. |
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