| 王建全,张亚娜,林敏.乙醇/硫酸铵双水相体系分离纯化款冬花总黄酮[J].井冈山大学自然版,2016,(1):40-45 |
| 乙醇/硫酸铵双水相体系分离纯化款冬花总黄酮 |
| SEPARATION AND PURIFICATION OF TOTAL FLAVONOIDS FROM TUSSILAGO FARFARA FLOWER WITH ETHANOL/AMMONIUM SULPHATE AQUEOUS TWO-PHASE SYSTEM |
| 投稿时间：2015-11-12 修订日期：2015-12-16 |
| DOI：10.3969/j.issn.1674-8085.2016.01.009 |
| 中文关键词: 款冬花 纯化 总黄酮 双水相 |
| 英文关键词: Tussilago farfara purification flavonoids aqueous two-phase system |
| 基金项目:甘肃省高校河西走廊特色资源利用省级重点实验室科研项目(XZ1203) |
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| 中文摘要: |
| 以低温烘干的款冬花为原料,利用乙醇/硫酸铵双水相体系分离纯化款冬花总黄酮。首先对无水乙醇、(NH4 )2 SO4、黄酮粗提取液、NaCl质量分数和pH值进行单因素研究,然后利用 Box-Benhnken Design 实验对(NH4)2SO4 质量分数、无水乙醇质量分数、黄酮粗提取液质量分数、pH 值进行了优化;结果表明:(NH4)2SO4质量分数为15.5%、无水乙醇质量分数为32.2%、黄酮粗提取液质量分数为20.4%、pH = 4.1时款冬花总黄酮萃取率可达99.21%,与预测的99.44%非常接近。 |
| 英文摘要: |
| Purification and isolation of flavonoids from traditional Chinese medicine is an important technology in biological research. In the present study, purification of flavonoids of Tussilago farfara flower were carried out simultaneously by using an aqueous two-phase system of ethanol/ammonium sulphate. The effects of crude extract concentration, (NH4)2SO4 amount, pH and ethanol amount on the extraction efficiency of total flavonoids were explored by single factor and Box-Behnken design methods. The optimal aqueous two-phase extraction conditions were mass crude extract 20.4%,pH 4.1,(NH4)2SO4 mass fraction 15.5%, ethanol mass fraction 32.2%. The highest extraction rate was up to 99.21%,which was very close to the prediction of 99.44%.This method is simple, easy to implement, using anhydrous ethanol toxicity, environmentally friendly. |
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