| 刘亮,丁丽娜,陈东林,冯华,周英信.天冬总皂苷的超声提取工艺优化[J].井冈山大学自然版,2015,(2):81-86 |
| 天冬总皂苷的超声提取工艺优化 |
| ULTRASONIC EXTRACTION OPTIMIZATION OF TOTAL SAPONINS FROM ASPARAGUS COCHINCHINENSIS (LOUR.) MERR. |
| 投稿时间：2015-01-09 修订日期：2015-03-01 |
| DOI：10.3969/j.issn.1674-8085.2015.02.017 |
| 中文关键词: 天冬 总皂苷 超声提取 |
| 英文关键词: Asparagus cochinchinensis total saponins ultrasonic extraction |
| 基金项目:遵义市科技局、遵义医药高等专科学校联合科技计划项目(遵市科合社字[2013]24号);遵义市汇川区科技计划项目。 |
| 作者 | 单位 | E-mail | | 刘亮 | 遵义医药高等专科学校, 贵州, 遵义 563002 | jikman1@163.com | | 丁丽娜 | 遵义医药高等专科学校, 贵州, 遵义 563002 | | | 陈东林 | 遵义医药高等专科学校, 贵州, 遵义 563002 | | | 冯华 | 遵义市药品检验所, 贵州, 遵义 563000 | | | 周英信 | 遵义医药高等专科学校, 贵州, 遵义 563002 | |
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| 中文摘要: |
| 目的 探讨天冬总皂苷含量的测定方法,优化超声提取工艺。方法 以菝葜皂苷元为对照品,采用紫外分光光度法测定总皂苷的含量,用单因素实验和正交实验优化超声提取工艺。结果 菝葜皂苷元质量浓度在5.2~52 μg/mL之间线性关系良好(r = 0.9994),总皂苷平均回收率达到98.83%(RSD = 1.88%)。最佳超声提取工艺为乙醇浓度50%、料液比1:20(g/mL)、提取温度60 ℃、提取时间20 min。结论 总皂苷含量测定方法简单、易行、准确,超声提取工艺高效、快捷,易于推广。 |
| 英文摘要: |
| Objective: To develop a method for determining the content of total saponins in Asparagus cochinchinensis and optimize its ultrasonic extraction process. Methods: Sarsasapogenin as the control sample, the content of total saponins was determined by ultraviolet spectrophotometry. Optimization of the ultrasonic extraction process of total saponins was performed using single factor and orthogonal array design methods. Results: The calibration curve of the sarsasapogenin was linear (r = 0.9994) in the range of 5.2~52 μg/mL with average spike recovery of 98.83% (RSD = 1.88%). The optimum ultrasonic extraction conditions were found to be: ethanol concentration of 50%, solid-to-liquid ratio of 1:20, extraction temperature of 60℃, ultrasonic extraction duration of 20 minutes. Conclusion: The developed method for total saponins determination is simple, feasible and accurate. The optimized extraction process is efficient and quick, and easy to popularize. |
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