曾建军,李军,李晓红,胡雪华.金线莲增殖培养研究[J].井冈山大学自然版,2013,(3):34-36 |
金线莲增殖培养研究 |
OPTIMIZATION FOR SHOOT PROLIFERATION OF ANOECTOCHILUS FORMOSANUS |
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DOI: |
中文关键词: 金线莲 正交设计 腋芽 增殖 |
英文关键词: Anoectochitusforrnosanus Hay orthogonal design shoot proliferation |
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中文摘要: |
通过正交设计方法L9(34)研究了培养基中的植物生长调节剂6-BA、NAA对金线莲(Anoectochitus formosanus Hay)茎段不同部位的腋芽增殖的影响。实验结果表明:最优水平组合为MS+6-BA3.0mg·L-1+NAA0.1mg·L-1,最适外植体为中部茎段。BA及茎段不同部位对实验结果的影响达到极显著水平(P≤0.01),该因素不同水平间差异达到极显著水平(P≤0.01)。 |
英文摘要: |
By applying orthogonal designed experiment L9(3^4), the effects of 6-BA, NAA in the culture medium on the proliferation of the axillary buds in different stem parts of Anoectochitusformosanus Hay were studied. The results indicate that the best combination is MS+6-BA 3 mg·L^-1+ NAA 0.1 mg·L^-1, the optimal explants are middle stems. BA and different stem segments influenced the results significantly (P ≤0.01), and differences among the three concentration levels are highly significant ( P≤ 0.01). |
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