| 罗辉,贺俐,吴杨.实时荧光定量PCR检测疫霉侵染下辣椒ERF基因的差异表达[J].井冈山大学自然版,2011,(5):127-131 |
| 实时荧光定量PCR检测疫霉侵染下辣椒ERF基因的差异表达 |
| THE DIFFERENTIAL EXPRESSION OF THE ERF GENE IN PEPPER(CAPSICUM ANNUUM L.) UNDER PHYTOPHTHORA CAPSICI STRESS |
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| DOI： |
| 中文关键词: 疫霉 ERF基因 实时定量PcR |
| 英文关键词: Phytophthora capsici ERF gene real-time quantitative PCR |
| 基金项目:国家自然科学基金项目(31060263); 江西省自然科学基金项目(2009GQN0077,2010GQN0118); 江西省教育厅科技计划项目(GJJ11537) |
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| 中文摘要: |
| 本研究利用real-tinle荧光定量PcR技术检测辣椒疫霉菌（Phytophthora capsici．）胁迫下辣椒抗病基因ERF的mRNA转录水平变化。结果表明：与对照相比，疫霉侵染下辣椒倒矿基因的表达量随着胁迫时间延长呈现出先升高后降低的变化特点，该基因在早期被诱导表达，在胁迫6h后表达量达到最大值，之后逐渐下降，相对表达量变化范围为2．83～31．07。 |
| 英文摘要: |
| The differential expression of ERF gene in pepper under Phytophthora capsici Stress was analyzed by real-time quantitative PCR.The results indicated that comparing with control,ERF gene transcription level in pepper leaves follows a high-low pattern under Phytophthora capsici Stress,ERF gene mainly expressed in the early stage of stress,reaching the peak 6 hours after treatment,and then decreased gradually.The relative expression ratios of the ERF gene ranged from2.83 to 31.07. |
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