| 褚洪标,胡有生,彭才圣,梁兆昌,刘永青.马血清破伤风抗毒素分离纯化的研究[J].井冈山大学自然版,2011,(4):98-100 |
| 马血清破伤风抗毒素分离纯化的研究 |
| SEPARATION AND PURIFICATION OF HORSE SERUM TETANUS ANTITOXINS |
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| DOI： |
| 中文关键词: 破伤风抗毒素 凝胶色谱 G-150 分离 纯化 |
| 英文关键词: tetanus antitoxins gel chromatography G-150 separation purification |
| 基金项目:吉安市科技局重点科技计划项目(吉市科计字[2009]40) |
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| 中文摘要: |
| 目的提高马血清破伤风抗毒素制剂中有效成分的含量。方法在超滤浓缩之后,利用凝胶色谱对原液进行纯化,利用电泳图谱对结果进行分析。结果葡聚糖凝胶(Sephadex G-150)对抗毒素的分离有较好效果,可去除一部分杂蛋白,提高F(ab)2等有效成分的含量。结论利用凝胶色谱对抗毒素进行分离可提高制品质量,降低不良反应,因此增加该工艺是可行的。 |
| 英文摘要: |
| Objective:To improve the content of active constituents in tetanus antitoxins.Methods:After ultrafiltration,the stock solution was purified by gel chromatography method and the results were analyzed by electrophoresis.Results:The contents of F(ab)2 were improved by use of Sephadex G-150 and the impurity proteins were decreased.Conclusion:The product quality was promoted by gel chromatography and the seperation technics may be feasible. |
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