| 朱立成,邹小明,刘江华,王忠先.三唑磷降解菌的筛选、鉴定及其系统发育分析[J].井冈山大学自然版,2011,(1):45-49 |
| 三唑磷降解菌的筛选、鉴定及其系统发育分析 |
| ISOLATION,IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF TRIAZOPHOS BIODEGRADING BACTERIA |
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| DOI： |
| 中文关键词: 三唑磷 生物降解 假单胞菌属 |
| 英文关键词: Triazophos biodegradation Pseudonmonas |
| 基金项目:江西省教育厅科技项目(赣教技字[2007]322号); 江西省千名科技人员入园入企行动合作项目 |
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| 中文摘要: |
| 从长期施用有机磷农药的土壤中,以三唑磷为唯一碳源和能源,采用逐渐加量的驯化方式,分离纯化到2株对三唑磷有较好降解能力的细菌,命名为TAP-W和TAP-R。其中TAP-W菌革兰氏染色阴性,能够在30℃～40℃范围内和pH6.0～9.0范围内良好生长,其最适生长温度为35℃,最适pH为7.0。TAP-W菌在含0.1%三唑磷的无机盐培养基中（三唑磷浓度400mg/L）振荡培养72h后,对三唑磷降解率最高,达到65.9%。根据TAP-W菌株的形态特征,生理、生化特性和系统发育分析,初步鉴定其为假单胞菌属（Pseudonmonas）细菌。 |
| 英文摘要: |
| Two Triazophos biodegradation bacteria strains named as TAP-W and TAP-R were isolated from the soil samples obtained in fields with frequent application in organ-phosphorus pesticides.TAP-W is a gram-negative bacterium,can grow well at 30～40 ℃ and pH 6.0～9.0,with the optimum temperature of 35 ℃ and pH of 7.0.After cultured in salt medium with 0.1% Triazophos（400mg/L） at a shaker（150 rpm） for 72 hours,TAP-W had the highest biodegradation rate of 65.9%.Based on the morphological,physiological and biochemical properties and phylogenetic analysis according to its 16S rRNA gene sequences,the TAP-W strain was identified as the genus Pseudonmonas. |
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